Figure 1.

Multiple particle tracking in ex vivo hemispheric brain slices. (A) Following euthanasia, brains were extracted, split into hemispheres sagittally, and sectioned coronally before being plated on cell culture inserts and cultured at 37 °C and 5% CO₂. Slices were allowed to rest overnight. (B) Forty nanometer PS–PEG nanoparticles were injected into the cortex and allowed to incubate for 30 min prior to video acquisition via high-speed fluorescence microscopy. Videos were acquired 20–24 h postslice preparation. (C) Videos were analyzed in ImageJ to track nanoparticle Brownian motions and quantify mean squared displacements and effective diffusion coefficients. Created with BioRender.com.