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. 2021 Jun 30;22(3):927. doi: 10.3892/etm.2021.10359

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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B3GALT5-AS1 silencing and CSNK2A1 overexpression on MKN-cells. (A) Expression levels of B3GALT5-AS1 in MKN-45 cells following transfection with shRNA-NC, shRNA-B3GALT5-AS1-1 or shRNA-B3GALT5-AS1-2 were determined by RT-qPCR. (B) Western blot analysis showing the protein expression levels of CSNK2A1. ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. shRNA-NC. (C) mRNA expression levels of CSNK2A1 following transfection with ov-NC or ov-CSNK2A1 were assessed by RT-qPCR. (D) Western blot analysis showing the protein expression levels of CSNK2A1 following transfection of MKN-45 cells with ov-NC or ov-CSNK2A1. ^**P<0.01 and ^***P<0.001 vs. ov-NC. B3GALT5-AS1, β-1,3-galactosyltransferase 5-AS1; CSNK2A1, casein kinase 2 a1; NC, negative control; RT-qPCR, reverse transcription-quantitative PCR; LncRNA, long non-coding RNA; Ov, expression; shRNA, short hairpin RNA.