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. 2021 Jul 15;27:77. doi: 10.1186/s10020-021-00332-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Overexpressed GSK3β inhibits protective effects of Propofol on H/R cells. A Autophagy-related protein expression in cells as detected by Western blot analysis (*p < 0.05, compared with that of the control group; ^#p < 0.05, compared with the H/R group). B Autophagosome amount in cells as measured by immunofluorescence against LC3 (*p < 0.05, compared with that of the control group; ^#p < 0.05, compared with the H/R group). C Inflammatory factor levels in cells as detected by ELISA (*p < 0.05, compared with the control group; ^#p < 0.05, compared with the H/R group). D GSK3β overexpression efficiency as detected by Western blot analysis. E, Expression of GSK3β and autophagy-related protein levels as determined by Western blot analysis (*p < 0.05, compared with that of the H/R + oe-NC group; ^#p < 0.05, compared with the H/R + oe-NC + Propofol group). F Autophagosome amount in cells as measured by immunofluorescence against LC3 (*p < 0.05, compared with the H/R + oe-NC group; ^#p < 0.05, compared with the H/R + oe-NC + Propofol group). G Cell apoptosis as assessed by flow cytometry (*p < 0.05, compared with the H/R + oe-NC group; ^#p < 0.05, compared with the H/R + oe-NC + Propofol group). H, Inflammatory factor levels as determined by ELISA (*p < 0.05, compared with the H/R + oe-NC group; ^#p < 0.05, compared with the H/R + oe-NC + Propofol group). The experiment was repeated 3 times independently