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. 2021 Jul 15;28:53. doi: 10.1186/s12929-021-00749-z

Fig. 5.

Fig. 5

Silenced NEAT1 inhibits ovarian granulosa cell apoptosis in PCOS rats. A TUNEL assay was used to detect the apoptosis of ovarian granulosa cells in PCOS rats after down-regulation of NEAT1 (Scale bar = 50 μm); B Apoptotic index of ovarian granulosa cells in PCOS rats after down-regulation of NEAT1; C Protein bands of Cleaved caspase-3, caspase-3, Bax and Bcl-2 in PCOS rats after down-regulation of NEAT1. D Cleaved caspase-3/caspase-3 protein expression in PCOS rats after down-regulation of NEAT1 detected by Western blot analysis. E Bax/Bcl-2 protein expression in PCOS rats after down-regulation of NEAT1 detected by Western blot analysis; n = 8. The data are all measurement data, expressed as mean ± standard deviation, *P < 0.05 vs. the normal group; #P < 0.05 vs. the sh-NC group, and one-way ANOVA and Tukey’s post hoc test were used for statistical analysis