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. Author manuscript; available in PMC: 2021 Oct 4.
Published in final edited form as: J Periodontol. 2021 Jan 6;92(10):1483–1495. doi: 10.1002/JPER.20-0529

FIGURE 2.

FIGURE 2

Senescence-associated factors interact with LPS. A) Jawbone cells isolated from 6-month-old mice were differentiated into osteocytes, by repeatedly exposed to LPS (10 μg/mL). CM from these senescent and healthy osteocytes was collected and analyzed by a bead-based multiplex cytokine array. Six replicates were used for each condition and protein concentration displayed in pg/mL. B) To evaluate the potential interaction between senescence-associated factors and LPS, differentiated osteocytes were exposed to media plus control CM (control), LPS (10 μg/mL) or LPS combined with SCM for 48 hours. The effect of SB202190, a selective p38 MAPK inhibitor (10 μM), was also evaluated by pretreating cells before starting the experiments. Data represent mean ± SEM. Significant changes are indicated by: * compared with control, † LPS compared with LPS+SCM, and ‡ LPS+SCM compared with LPS+SCM+SB202190. Various levels of significance are based on the number of each respective symbol (one symbol P < 0.05; two symbols P < 0.01; three symbols P < 0.001)