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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Exp Neurol. 2021 Jan 19;338:113603. doi: 10.1016/j.expneurol.2021.113603

Figure 5.

Figure 5

Rotarod, open-field task and fear conditioning tests in male (A-D) and female (E-H) at 10 to 12-month-old mice. A, Accelerating rotarod test was performed in male (A) and female (E). In male, there was no difference in the latency to fall between KO and CON mice (A), unpaired t test, t statistic [df: 18] =0.3326, p-value=0.7433, n=10 each for CON and KO mice. In female, KO mice showed a decline supporting a sooner fall from the rod than CON mice (E), unpaired t test, t statistic [df: 18] =1.638, p-value=0.1188, n=10 each for CON and KO mice. B-C & F-G, Open-field tasks were performed and the total path length within the whole arena (B & F) and distance traveled in center of the arena (C & G) are shown. Distance traveled in the whole arena (B & F) reflected the KO mice covering greater total path length of the whole arena compared to CON by both male (B) and female (F). In male, unpaired t test with Welch’s correction, t statistic [df: 17.98] =3.813, *p-value=0.0013, n=10 each for CON and KO mice; In female, unpaired t test with Welch’s correction, t statistic [df: 10.83] =2.261, *p-value=0.0454, n=10 each for CON and KO mice. Distance traveled in the center of arena (C & G), while male KO mice covered less distance in the center compared to CON mice, no significant difference was noted (C), unpaired t test with Welch’s correction, t statistic [df: 16.20]=2.091, p-value=0.0526, n=10 for both CON and KO mice. Female KO mice travelled less distance in the center of the arena and demonstrated tendencies in staying near the corners (G), unpaired t test with Welch’s correction, t statistic [df: 13.87]=2.399, *p-value=0.0311, n=10 for both CON and KO mice. D & H, Contextual fear conditioning, Although male KO mice showed a similar impaired fear conditioning pattern with no significant difference (D), two-way ANOVA followed by Sidak’s multiple-comparisons test.: interaction, F statistic [df:1,36]=2.436, p=0.1273; day1 to day 2, F statistic [df: 1, 36]=187.4, p<0.0001; genotype, F statistic [df:1, 36]=2.01, p=0.1648. p-value for day 2=0.0827, n=10 for both CON and KO), female KO mice showed impaired contextual fear conditioning. KO mice spent significantly less time freezing compared to CON (H), two-way ANOVA followed by Sidak’s multiple-comparisons test.: interaction, F statistic [df:1,36]=5.271, p=0.0276; day1 to day 2, F statistic [df: 1, 36]=164.1, p<0.0001; genotype, F statistic [df:1, 36]=6.33, p=0.0165. *p-value for day 2=0.0033, n=10 for both CON and KO.