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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Exp Neurol. 2021 Jan 19;338:113603. doi: 10.1016/j.expneurol.2021.113603

Figure 6.

Figure 6

Water maze and social interaction tests were performed in male (A-C) and female (D-F) 10 to 12-month-old mice. A & D, Time spent in each quadrant (T, target quadrant; L, left quadrant; O, opposite quadrant; R, right quadrant) was measured in the probe trial in which the platform was removed from the pool. In male (A), glut3 KO mice spent less time in target quadrant than CON although no significant difference was detected. glut3 KO mice spent more time in opposite quadrant that did not achieve statistical significance, two-way ANOVA followed by Sidak’s multiple-comparison test; interaction, F statistic [df: 3, 72]=4.216, p=0.0084; Time spent, F statistic [df:3,72]=27.58, p<0.0001; genotype, F statistic [df:1, 72]=1.696e-006, p=0.9990. p=0.6190 for target quadrant; p=0.0951 for left quadrant; p=0.7209 for opposite quadrant; p=0.1345 for right quadrant. Female (D), glut3 KO mice spent significantly less time in target quadrant than CON, with more time spent in the opposite quadrant, two-way ANOVA followed by Sidak’s multiple-comparisons test; interaction, F statistic [df: 3, 72]=6.662, p=0.0005; Time spent around, F statistic [df:3,72]=19.18, p<0.0001; genotype, F statistic [df:1, 72]=0.0009763, p=0.9752. *p=0.0342 for target quadrant; p=0.6887 for left quadrant; #p=0.0056 for opposite quadrant; p=0.9606 for right quadrant. B & E. Sociability test in male (B) and female (E) mice revealed that while both male and female KO mice spent more time compared to respective CON with the novel mouse than with the empty wire cup, only females achieved statistical significance. In male, two-way ANOVA followed by Sidak’s multiple comparison test: interaction, F statistic [df: 1, 36]=3.835, p=0.0580; time spent around, F statistic [df: 1, 36]=0.0002, p=0.6270; genotype, F statistic [df: 1, 36]=8.912, p=0.2999; n=10 for both CON and KO. In KO, Empty vs Stranger p=0.2183; in female, two-way ANOVA followed by Sidak’s multiple comparison test: interaction, F statistic [df: 1, 36]=0.3211, p=0.5744; time spent around, F statistic [df: 1, 36]=0.1624, p=0.6893; genotype, F statistic [df: 1, 36]=12.47, p=0.0012; n=10 for both CON and KO *p=0.0376. C & F. Social novelty test conducted in male (C) and female (F) mice, the male glut3 KO mice were no different from CON in time spent with novel mouse or familiar mouse (C), two-way ANOVA followed by Sidak’s’s multiple comparison test; interaction, F statistic [df:1,36]=0.6379, p=0.4297; Time spent around, F statistic [df: 1, 36]=2.849, p=0.1001: genotype, F statistic [df: 1, 6]=0.6818, p=0.4144; n=10 for both CON and KO. Female glut3 KO mice spent significantly more time with the novel mouse rather than the familiar mouse (F) (unfamiliar versus familiar of CON, p=0.2092), two-way ANOVA followed by Sidak’s’s multiple comparison test; interaction, F statistic[df:1,36]=0.3729, p=0.5453; Time spent around, F statistic [df: 1, 36]=0.2178, p=0.6435: genotype, F statistic [df: 1, 36]=13.33, p=0.0008; n=10 for both CON and KO. Unfamiliar versus familiar in the case of glut3 KO, *p=0.0279