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. 2021 Jul 1;22(3):944. doi: 10.3892/etm.2021.10376

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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TG reverses the anti-apoptotic effect of rutin on H9C2 cells induced by high glucose. (A) Cell viability was determined by CCK-8. (B) LDH kit was used to determine cytotoxicity. (C) Tunel assay determined the apoptosis level of cells. (D) Quantitative analysis of the apoptosis level in cells. (E) Western blot analysis was used to determine the expression levels of apoptosis related proteins. TG, thapsigargin; CCK-8, cell counting kit-8; LDH, lactate dehydrogenase; HG, high glucose; NG, normal glucose; MA, mannitol. ^**P<0.01, ^***P<0.001 vs. MA. ^#P<0.05, ^##P<0.01, ^###P<0.001 vs. HG. ^ΔP<0.05, ^ΔΔΔP<0.001 vs. HG + Rutin.