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. Author manuscript; available in PMC: 2021 Jul 15.
Published in final edited form as: Annu Rev Physiol. 2020 Oct 26;83:183–203. doi: 10.1146/annurev-physiol-031620-091043

Figure 5.

Figure 5

CaV1.2 is expressed during mouse limb development. (a) A CaV1.2lacZ/+ reporter mouse detects CaV1.2 expression during mouse limb development. A frozen tissue section (10 μM) from CaV1.2lacZ/+ forelimb at E14.5 was stained with X-gal and counterstained with nuclear fast red. (b) Normal skeletal development of Col2a1-Cre;Cacna1cflox/flox conditional knockout (cKO) mouse embryos. Double staining with alizarin red and Alcian blue of the whole skeleton (top), hindlimbs and forelimbs (bottom) of Cacna1cflox/flox (control), and cKO littermate mouse embryos at P0.