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. 2021 Jul 15;11:132. doi: 10.1186/s13578-021-00649-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — YTHDF2 protein expression increases in the heart tissues of mice with cardiac hypertrophy. A RT-PCR analysis of YTHDF1/2/3 mRNA expressions in the primary cardiomyocytes stimulated with isoproterenol (ISO, 10 μmol/l), phenylephrine (PHE, 50 μmol/l), or DMSO (as control) for 24 h. B Western blotting analysis of YTHDF1/2/3 protein expressions in the primary cardiomyocytes stimulated with ISO (+ , 10 μmol/l; + + , 20 μmol/l), PHE (+ , 50 μmol/l; + + , 80 μmol/l), or DMSO (as control) for 24 h. C Densitometry quantification of protein expressions. D RT-PCR analysis of YTHDF1/2/3 mRNA expressions in the heart tissues of mice after 4 weeks of TAC surgery (n = 6) or Sham surgery (n = 6). E Western blotting analysis of YTHDF1/2/3 protein expressions in the heart tissues of Sham (n = 6) or TAC (n = 6) mice. F Densitometry quantification of protein expressions. G WGA staining (Green) was used to measure cardiomyocyte size in the heart tissues of Sham or TAC mice; Immunofluorescence analysis of YTHDF1/2/3 (Red) expressions in cardiomyocytes (stained with anti-α-actinin antibodies, Green). Nucleus was stained with DAPI (Blue). ^*P < 0.05