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. Author manuscript; available in PMC: 2021 Jul 15.
Published in final edited form as: Virology. 2019 Aug 29;537:246–253. doi: 10.1016/j.virol.2019.08.026

Figure 1. pTRS1 stimulates poliovirus IRES activity.

Figure 1.

(A) Bicistronic mRNA reporter schematic. IRES activity is defined as the level of firefly luciferase (FLuc) compared to the level of renilla luciferase (RLuc). (B) HeLa cells were co-transfected with a bicistronic reporter containing no IRES element (EV; empty vector) or a reporter containing the poliovirus IRES (Polio) in the presence of GFP or TRS1. The average fold change of RLuc (black bars) and Fluc (grey bars) compared to EV co-transfected with GFP is shown (n=3). (C) As in (B), fold change in EV and Polio IRES activity in the presence of pTRS1 compared to GFP. *(p<0.05); **(p<0.01)