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. Author manuscript; available in PMC: 2021 Jul 15.
Published in final edited form as: Virology. 2019 Aug 29;537:246–253. doi: 10.1016/j.virol.2019.08.026

Figure 2. pTRS1 enhances virus IRES activity.

Figure 2.

HeLa cells were co-transfected with a bicistronic reporter containing no IRES element (EV), the KSHV vFLIP IRES (KSHV) or the poliovirus IRES (Polio) and either GFP or TRS1. Average fold change in RLuc (A), FLuc (B) and IRES activity (C) in cells transfected with TRS1 compared to cells transfected with GFP is shown for each reporter (n=3). (D) RNA was extracted from cells transfected as in (A). RLuc (black bars) and Fluc (grey bars) RNA levels were determined by RT-qPCR. Average fold change in RLuc and FLuc RNA in cells transfected with TRS1 compared to cells transfected with GFP is shown for each bicistronic reporter (n=2). *(p<0.05); **(p<0.01); ***(p<0.005)