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. Author manuscript; available in PMC: 2021 Jul 15.
Published in final edited form as: Virology. 2019 Aug 29;537:246–253. doi: 10.1016/j.virol.2019.08.026

Figure 5. pTRS1 stimulation of IRES activity is independent of PKR antagonism.

Figure 5.

(A) Control HeLa or PKR KO HeLa cells were co-transfected with the a bicistronic reporter containing no IRES (EV), the KSHV vFLIP IRES (KSHV), or the poliovirus IRES (Polio) and GFP. Average IRES activity of each reporter (FLuc/RLuc) (n=3) is shown. (B) PKR KO HeLa cells were co-transfected with the a bicistronic reporter containing no IRES (EV), the KSHV vFLIP IRES (KSHV), or the poliovirus IRES (Polio) with either GFP (black bars) or TRS1 (white bars). Average IRES activity of each reporter (FLuc/RLuc) (n=3) is shown. (C) WT and PKR KO HeLa cells were co-transfected with a circular GFP reporter containing the KSHV vFLIP IRES and either a vector control or TRS1, anf GFP levels were meaureed by Western blot. A representivate image showing GFP and pTRS1 expression is shown (n=3). Numbers indicate the fold change in GFP expression compared to vector control, normalized to pTRS1 and tubulin levels. *(p<0.05); **(p<0.01)