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. 2021 Jul 1;24(7):102806. doi: 10.1016/j.isci.2021.102806

Figure 1.

Figure 1

Detection of Olig2-AS in aldh1L1-GFP/olig2-tdTomato double transgenic mice

(A–D) Here and in all subsequent panels, images show transverse sections of hemi-ventral spinal cord. (A–d3) Combined detection of eGFP (green), td-Tomato (red), and Olig2 (blue, A–A2; C–c3) or Sox10 (blue, B–B2, D–d3) on aldh1L1-eGFP/olig2-tdTomato transgenic mice at E18.5 (A–B2) and P7 (C–d3). Horizontal sets in A–B2 present successively GFP and tdTomato staining, Olig2 (A1) or Sox10 (B1) and GFP staining, and the merged image. Images (c–c3) and (d–d3) show higher magnification of the areas framed in (C and D), respectively, and show successively tdTomato (C and D) and eGFP (c1 and d1) staining, double tdTomato and eGFP staining (c2 and d2), and Olig2 (c3) or Sox10 (d3) and eGFP staining. Colored arrows point to OPC/OL (yellow), Olig2-AS (white), and nonOlig2-AS (blue). Scale bars, 100 μm in (C and D); 50 μm in (A–A2) and (B–B2); and 25 μm in (c–c3) and (d–d3).

See also Figures S1 and S2.