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. 2021 Jul 1;24(7):102806. doi: 10.1016/j.isci.2021.102806

Figure 4.

Figure 4

The up-regulated genes inka2 and kcnip3 are specifically expressed in Olig2-AS

(A–H) (A and C) Expression profiles of inka2 (A) and kcnip3 (C) mRNAs at P7. (B and D) Immunodetection of Olig2 (green) combined with detection of fgfr3 (red) and inka2 (gray, B) or kcnip3 (gray, D) mRNAs in the ventral spinal cord at P7. (E–H‴) (E–F‴) and (G–H‴) show higher magnification of the areas framed in (B and D), respectively, and show successively combined Olig2 immunostaining and detection of fgfr3 mRNA (E, F, G, H), combined detection of fgfr3 and inka2 (E′, F′) or kcnip3 (G′, H′) mRNA, combined Olig2 immunostaining and detection of inka2 (E″, F″) or kcnip3 mRNA (G″, H″), and the merged images (E‴, F‴, G‴, H‴). Colored arrows point to OPC/OL (yellow), Olig2-AS (white), and nonOlig2-AS (blue). Scale bars, 100 μm in (A and C), 50 μm in (B and D), and 25 μm in (E–H‴).

See also Figures S8 and S9.