REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-Olig2 antibody	Millipore	Cat#AB9610; RRID: AB_570666
Goat anti-Sox10 antibody	Santa Cruz Biotechnology	Cat#sc-17342; RRID: AB_2195374
AP conjugated anti-DIG antibody	Sigma-Aldrich	Cat#11093274910; RRID: AB_2734716
POD conjugated anti-Fluo antibody	Sigma-Aldrich	Cat#11426346910; RRID: AB_840257
Donkey anti-rabbit IgG AlexaFluor-488	ThermoFisher Scientific	Cat#A-21206; RRID: AB_2535792
Donkey anti-goat IgG AlexaFluor-488	ThermoFisher Scientific	Cat#A-11055; RRID: AB_2534102
Donkey anti-rabbit IgG AlexaFluor-647	ThermoFisher Scientific	Cat#A-31573; RRID: AB_2536183
Donkey anti-goat IgG AlexaFluor-647	ThermoFisher Scientific	Cat#A-21447; RRID: AB_141844
AlexaFluor 488 Tyramide kit	ThermoFisher Scientific	Molecular Probes Cat#B-40912
AlexaFluor 555 Tyramide kit	ThermoFisher Scientific	Molecular Probes Cat#B-40913
Chemicals, peptides, and recombinant proteins
Paraformaldehyde	Sigma-Aldrich	Cat#158127
Phosphate-buffer saline (PBS)	Sigma-Aldrich	Cat#D1408
Sucrose	Sigma-Aldrich	Cat#S0389
OCT media	Sakura	Cat#4583
Triton X-100	Sigma-Aldrich	Cat#T8787
Tween-20	Euromedex	Cat#2001-A
Sheep serum	Sigma-Aldrich	Cat#S2263
FBS	ThermoFisher Scientific	Gibco Cat#A31604-01
Superfrost Plus microscope slides	Thermoscientific	Cat#J1800AMNT
40μm cell strainer	Falcon	Cat#352340
NBT	Sigma-Aldrich	Cat#10760994001
BCIP	Sigma-Aldrich	Cat# N6639
Trizol	Thermofisher Scientific	Cat#15596026
RNase/DNase-free water	Bio-RAD	Cat#W4502
RNasin	Roche	Cat#N2511
DTT	Promega	Cat#P1171
Evagreen	Bio-RAD	Cat# 1725204
Critical commercial assays
Papain dissociation system	Worthington Biochemical	Cat#LK003150
Deposited data
Raw and analyzed data	This paper	GEO: GSE158517
Experimental models: Organisms/strains
Mouse: Tg(Aldh1l1-eGFP/Rpl10a) JD130Htz	GENSAT, Kindly provided by Dr. N. Rouach, Paris, FRANCE	RRID: IMSR_JAX:030247
Mouse: Olig2-tdtomato	GENSAT, Tg(Olig2-tdTomato)TH39Gsat	MGI: 5311714 http://www.informatics.jax.org/allele/MGI:5311714#imsr
Oligonucleotides
Primer for genotyping GFP-Fw	This paper	5’-CGCACCATCTTCTTCAAGGACGAC-3’
Primer for genotyping GFP-Rev	This paper	5’-AACTCCAGCAGGACCATGTGATCG-3’
Primer for genotyping Tomato-Fw	This paper	5’-CTGTTCCTGTACGGCATGG-3’
Primer for genotyping Tomato-Rev	This paper	5’-GGCATTAAAGCAGCGTATCC-3’
Primers for qPCR	See Table S5	N/A
Software and algorithms
STAR aligner	Dobin et al., 2012	https://github.com/alexdobin/STAR
FastQC	Babraham Bioinfomatics	RRID:SCR_014583 https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
HTSeq count	Anders et al., 2014	https://htseq.readthedocs.io/en/master/
DeSeq2	R-Bioconductor (Love et al., 2014)	RRID:SCR_015687 https://bioconductor.org/packages/release/bioc/html/DESeq2.html
Bioconductor (3.10)	R-Bioconductor	www.biocondutor.org
GOsummaries	R-Bioconductor (Kolde and Vilo, 2015)	https://www.bioconductor.org/packages/release/bioc/html/GOsummaries.html
Revigo	(Supek et al., 2011)	RRID:SCR_005825 http://revigo.irb.hr/
ggplot2	CRAN (Wickham, 2016)	RRID:SCR_014601 https://ggplot2.tidyverse.org
GOplot	CRAN (Walter et al., 2015)	https://wencke.github.io/
Cufflinks (v2.2.1)	Trapnell et al., 2010	RRID:SCR_014597 http://cole-trapnell-lab.github.io/cufflinks
Heatmap.2	R-Bioconductor
ImageJ	NIH	https://imagej.nih.gov
Leica LAS X	Leica systems	RRID:SCR_013673 https://www.leica-microsystems.com
Prism8	Graphpad Software	RRID:SCR_002798 https://www.graphpad.com/
Affinity Photo	Serif	RRID:SCR_016951 https://affinity.serif.com
Affinity Designer	Serif	https://affinity.serif.com
R (3.6.0)	The R foundation	https://www.r-project.org/
Other
Cryostat CM1950	Leica	RRID:SCR_018061
Tissue-chopper	Mc Ilwain	RRID:SCR_015798
Nikon Eclipse 80i microscope	Nikon
Leica SP5 confocal	Leica
Leica SP8 confocal	Leica
CFX Maestro BioRad	Bio-RAD	Cat#185-5096