Figure 6.

ATF6 regulates angiogenesis-osteogenesis coupling through FGF2 in vivo

(A) Representative SKG mice 12 weeks after curdlan injection. Insets show a healthy, untreated SKG mouse (left), a curdlan-treated SKG mouse with arthritis (middle) and a curdlan-treated SKG mouse treated with Ceapin-A7.

(B) Clinical scores in SKG mice were measured (n = 10 mice per group).

(C) Local mRNA expression of TNF-α, IFN-γ and IL-17 in the hind paws of SKG mice. (n = 10 mice per group).

(D) IF staining of vertebral bodies from SKG mice showing endothelial cells (CD31+) and osteoblasts (OSX+). Scale bar, 50 μm.

(E) Quantification of the proportions of CD31 + vessels and OSX + cells (n = 10 mice per group).

(F and G) Representative micro-CT images of the spine (F) and ankle (G) obtained from SKG mice at baseline, 12 weeks after induction, and 12 weeks after induction and treated with Ceapin-A7. The red arrows point to bony bridging between vertebrae in (F) and osteophytes in (G). IHC staining of FGF2 in spine and ankle. Scale bar, 50 μm.

Bars show the means ± SD. ∗p < 0.05 and ∗∗p < 0.01, one-way ANOVA followed by Dunnett's post hoc test.