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. 2021 Jul 1;12:707404. doi: 10.3389/fimmu.2021.707404

Figure 2.

Figure 2

TEC- or DC-derived LTβR does not regulate thymic egress. (A, B) Flow cytometric analysis of SP4 thymocytes in K14 Cre Ltbr fl/fl and littermate control mice. (A) Representative dot plots are shown. (B) The graphs display the statistical analysis of the percentage of immature or mature CD4+ SP thymocytes among total SP4 population. Mean ± SEM; n = 3. Data are representative of at least three independent experiments. (C, D) Flow cytometric analysis of egressing CD4+ T cells in K14 Cre Ltbr fl/fl and littermate control mice. (C) Representative dot plots of IVCD4-labeled thymocytes are shown (left). Egressing CD4+ T cells are gated from IVCD4-labeled thymocytes (right). (D) The graphs display the statistical analysis of the percentage of egressing CD4+ T cells among total mSP4. Mean ± SEM; n = 3. Data are representative of at least three independent experiments. (E, F) Flow cytometric analysis of SP4 thymocytes in Cd11c Cre Ltbr fl/fl and littermate control mice. (E) Representative dot plots are shown. (F) The graphs display the statistical analysis of the percentage of immature or mature thymocytes among total SP4 population. Mean ± SEM; n = 3 and 5. Data are representative of two independent experiments. (G, H) Flow cytometric analysis of egressing CD4+ T cells in Cd11c Cre Ltbr fl/fl and littermate control mice. (G) Representative dot plots of IVCD4-labeled thymocytes are shown (left). Egressing CD4+ T cells are gated from IVCD4-labeled thymocytes (right). (H) The graphs display the statistical analysis of the percentage of egressing CD4+ T cells. Mean ± SEM; n = 3 and 5. Data are representative of at least three independent experiments. ns, P > 0.05 (unpaired Student’s t-test).