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. 2021 Jul 1;11:681530. doi: 10.3389/fonc.2021.681530

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Copyright © 2021 Fallah, Demas, Jin, He and Shajahan-Haq

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increased WEE1 correlates with survival of LTED cells and with poor prognosis in breast cancer. (A, B) Protein samples from MCF7 and T47D and their LTED derivatives (MCF7-LTED and T47D-LTED) were collected under basal conditions and fractionated to nuclear or cytoplasmic fractions and immunoblotted with indicated antibodies. Increased WEE1 protein levels was detected in both cytoplasm or nuclei of MCF7-LTED cells compared with the corresponding fractions in MCF7 cells. Similarly, in T47D-LTED cells, WEE1 protein levels were increased in cytoplasmic and nuclear fractions compared with the corresponding fractions in T47D cells. Total H2AX (nuclear fractions) and β-actin (cytosolic fractions) were used as controls. Blot represents one of three independent experiments. (C, D) Kaplan-Meier survival curves was generated using two databases (GSE2034; n=134, GSE7390; n=209). Higher WEE1 gene expression (red) correlated with reduced relapse free survival (rfs) compared with low WEE1 gene expression (blue).