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. 2021 Jul 1;11:681530. doi: 10.3389/fonc.2021.681530

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Copyright © 2021 Fallah, Demas, Jin, He and Shajahan-Haq

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of p53 enhances AZD1775 mediated inhibition of cell growth in parental MCF7 and T47D cells. (A, B) Parental MCF7 and T47D and their respective LTEDs were transfected with p53 siRNA or scramble negative control (ctrl) siRNA for 72h. Whole cell lysates were subjected to immunoblotting with p53 antibody to confirm knockdown; β-actin was used as protein loading control. (C–F) Following 24h post transfection, cells were treated with 500nM AZD1775 for another 48 h and cell number was measured using the crystal violet assay. ***p < 0.001, ****p < 0.0001 or ns (not significant) (ANOVA) for cell number with p53 siRNA+AZD1775 compared to Ctrl siRNA+AZD1775 for respective cell lines. All blots and proliferation assays are from three independent experiments (n=3).