Figure 4.

hBD2 inhibits HIV-1 in macrophages post-entry via G_i-protein-mediated signaling. MDM were infected with single-cycle HIV-luciferase virus pseudotyped with AMLV envelope followed by pretreatment with or without PTx (100 ng/ml). Cells were then incubated 3 days in presence or absence of hBD2. Subsequently, cells were lysed and luciferase activity was measured. Percentage of inhibition was calculated for treated infected cells in reference to untreated infected cells. Data are presented for independent experiments from 3 different donors.