Table 3.
Average of the methylation percentage (± SEM) in tumoral and control tissues along with the statistical results between the two groups and the ROC curve analysis.
| Gene | TUMORAL N = 18 | CONTROL N = 6 | % Methylation T vs C | ROC | |||||
|---|---|---|---|---|---|---|---|---|---|
| Mean | SEM | Mean | SEM | p-value (Mann-Whitney U test) | AUC | Cut-off% | Sensitivity%/ Specificity% | p-value | |
| RUNX3 | 78.87 | 4.05 | 48.31 | 4.83 | <0.001 | 0.917 | 55.57 | 94.4–83.3 | 0.003 |
| SFRP1 | 54.61 | 7.44 | 3.18 | 0.98 | <0.001 | 0.944 | 10.51 | 94.4–100 | 0.001 |
| SPARC | 52.01 | 6.14 | 33.66 | 4.78 | ns | ||||
| WIF1 | 51.95 | 7.13 | 6.66 | 1.69 | 0.003 | 0.889 | 15.69 | 88.9–100 | 0.005 |
| PCDH10 | 50.31 | 7.07 | 4.95 | 1.01 | 0.001 | 0.935 | 12.03 | 88.9–100 | 0.002 |
| HS3ST2 | 50.03 | 8.39 | 11.04 | 8.7 | ns | ||||
| DKK2 | 47.96 | 6.68 | 7.38 | 1.58 | <0.001 | 0.981 | 13.81 | 94.4–100 | 0.001 |
| DKK3 | 47.22 | 8.65 | 2.35 | 0.55 | 0.027 | 0.806 | 3.92 | 77.8–83.3 | 0.028 |
| TMEFF2 | 46.4 | 7.89 | 1.87 | 0.4 | 0.002 | 0.907 | 2.99 | 88.9–83.3 | 0.003 |
| OPCML | 43.28 | 5.37 | 9.38 | 2.69 | 0.003 | 0.889 | 15.20 | 83.3–83.3 | 0.005 |
| WT1 | 42.9 | 9.09 | 0.9 | 0.23 | ns | ||||
| SFRP2 | 41.84 | 7.51 | 2.18 | 0.66 | 0.015 | 0.833 | 10.84 | 83.3–100 | 0.016 |
| UCHL1 | 36.95 | 7.6 | 5.28 | 2.65 | ns | ||||
| HIC1 | 29.87 | 8.12 | 4.34 | 0.58 | ns | ||||
| SFRP5 | 26.87 | 7.57 | 2.53 | 0.93 | ns | ||||
| RASSF1 | 20.28 | 6.6 | 0.87 | 0.22 | ns | ||||
| CDKN2A | 19.03 | 7.09 | 1.84 | 0.38 | Methylation <20% | ||||
| MLH1 | 14.48 | 6.88 | 2.05 | 0.95 | |||||
| APC | 9.2 | 3.36 | 2.28 | 0.58 | |||||
| MGMT | 7.81 | 3.38 | 3.5 | 1.54 | |||||
| CDH1 | 1.08 | 0.1 | 7.35 | 2.38 | |||||
| HNF1B | 0.9 | 0.11 | 1.23 | 0.43 | |||||
The bold values refer to significant results.
NS, not significant.