Fig 4. Analysis of the virus titers in the brain and blood viral RNA levels in JCV-infected C57BL/6J mice treated with FPV and PBS.

Mice (4–6/group) were inoculated intracerebrally with 1.0×10⁴ TCID₅₀ JCV. FPV (300 mg/kg/day) and PBS treatments were initiated 2 days prior to virus inoculation (pre-treatment) or on the same day of virus inoculation (simultaneous-treatment) and continued for 5 days. The virus titers in the brain (a) and the blood RNA levels (b) in JCV-infected C57BL/6J mice, 1, 3, and 5 dpi are shown. The data are obtained from a single experiment. Each bar represents the geometric mean with the geometric standard deviation. The dotted line indicates the limit of detection of RT-qPCR. Asterisks denote significant differences (p < 0.05).