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. 2021 Jul 2;17(7):e1009132. doi: 10.1371/journal.pcbi.1009132

Fig 4. Validation of RNF43 G659fs pathway impact.

Fig 4

(A) Cignal 10-Pathway Reporter Array data obtained in HEK-293T cells upon overexpression of Vector, RNF43-WT, RNF43-R117fs and RNF43-G659fs. Each bar represents the mean±s.d. acquired from three independent experiments. A two-tailed Student’s t-test was used for statistical analysis (*P<0.05). (B) Western blot analysis upon overexpression of GFP, RNF43-WT, RNF43-R117fs, or RNF43-G659fs in HEK-293T of IκBα, p-Erk, Vimentin, HIF-1α and p-JNK. Anti-β-actin was used as a loading control (C) True positive rate (sensitivity) and false positive rate (1-specificity) across eVIP2, 80 variations of GSEA, and 8 variations of FGSEA.