Table 1. Genes with functions in Tetrahymena meiosis.
| Process TGD ID |
NCBI Gene ID, Protein ID |
Meiosis specific | Function | Budding yeast homolog | Reference(s) |
|---|---|---|---|---|---|
| Meiosis initiation and regulation | |||||
| TCDK3 (TTHERM_00483640) | Gene ID: 7840816, XP_001017477.2 | Y | Meiotic cyclin–dependent kinase | CDC28 | [17] |
| CYC2 (TTHERM_00079530) | Gene ID: 7831246, XP_001015793.1 | Y | Meiotic B-type cyclin | CLB4 | [16] |
| CYC17 (TTHERM_00693080) | Gene ID: 7827170, XP_001023573.2 | Y | Meiotic B-type cyclin | CLB2 | [20] |
| CYC28 (TTHERM_00082190) | Gene ID: 7845854, XP_001012564.3 | Y | Meiotic cyclin | — | [21] |
| E2FL1 (TTHERM_00695710) | Gene ID: 7837814, XP_001025582.2 | Y | Meiotic E2F family transcription factor | — | [18] |
| DPL2 (TTHERM_00047010) | Gene ID: 7844987, XP_001014696.1 | Y | Meiotic transcription factor | — | [19] |
| APRO1 (TTHERM_00112830) | Gene ID: 7843544, XP_001010678.3 | Y | Meiotic transcription factor | — | Unpublished |
| DSB formation | |||||
| SPO11 (gene_000008668)1 | Not listed | Y | Meiotic DSB formation | SPO11 | [25] |
| PARS11 (TTHERM_00133730) | Gene ID: 7829243, XP_001019652.3 | Y | Meiotic DSB formation and control | (REC114 ?)3 | [41] |
| PARS-L (TTHERM_00530039) | Gene ID: 24442767, XP_001470656.1 | Y | Meiotic DSB formation | — | Unpublished |
| Homologous pairing | |||||
| CNA1 (TTHERM_00146340) | Gene ID: 7840155, XP_001011273.1 | N | Centromeric histone, meiotic nuclear reorganization | CSE4 | [27] |
| MELG1 (TTHERM_00711850) | Gene ID: 7827803, XP_001025845.2 | Y | Meiotic nuclear reorganization | — | [23] |
| MELG2 (TTHERM_00289290) | Gene ID: 7846875, XP_001018646.3 | Y | Meiotic nuclear reorganization | — | [23] |
| MELG3 (gene_000025916)1 | QPL17970.1 | Y | Meiotic nuclear reorganization | — | [23] |
| Crossover, chiasma formation | |||||
| ATR1 (TTHERM_01008650) | Gene ID: 7843404, XP_001026698.2 | N | Meiotic DSB sensing | MEC1 | [24] |
| MRE11 (TTHERM_00721450) | Gene ID: 7841076, XP_001031877.2 | N | DSB end processing | MRE11 | [35,42] |
| COM1 (TTHERM_00460480) | Gene ID: 7824714, XP_001018744.1 | Y | DSB end processing | COM1/SAE2 | [35] |
| EXO1 (TTHERM_01179960) | Gene ID: 7846424, XP_001030028.2 | (Y)2 | DSB end processing | EXO1 | [42] |
| SGS1 (TTHERM_01030000) | Gene ID: 7824842, XP_001015163.2 | N | Helicase, processing of recombination intermediates | SGS1 | [48] |
| MUS81 (TTHERM_00624870) | Gene ID: 7831680, XP_001022614.2 | N | Double Holliday junction resolution | MUS81 | [48] |
| MMS4 (TTHERM_00194130) | Gene ID: 7844641, XP_001017166.1 | N | Partner of Mus81, double Holliday junction resolution | MMS4 | [48] |
| DMC1 (TTHERM_00459230) | Gene ID: 7828368, XP_001024231.1 | Y | Strand exchange with homologous chromosome | DMC1 | [36] |
| RAD51 (TTHERM_00142330) | Gene ID: 7827499, XP_001011071.1 | N | Regulates Dmc1 nucleoprotein filament formation | RAD51 | [36] |
| HOP2 (TTHERM_00794620) | Gene ID: 7825538, XP_001020981.3 | Y | Bivalent formation | HOP2 | [25] |
| MND1 (gene_000003690)1 | Not listed | Y | Bivalent formation | MND1 | [25] |
| BIME2 (TTHERM_00530659) | Gene ID: 7827388, XP_001470665.1 | Y | Promotes or stabilizes Dmc1 nucleoprotein filaments | (RAD54 ?)3 | [44] |
| MSH4 (TTHERM_00857890) | Gene ID: 7832209, XP_001021931.2 | Y | MutS-domain protein, stabilizes joint molecules | MSH4 | [37] |
| MSH5 (gene_000007168)1 | Not listed | Y | MutS-domain protein, stabilizes joint molecules | MSH5 | [37] |
| ZHP3 (TTHERM_00049220) | Gene ID: 7830970, XP_001014817.4 | Y | Stabilizes D-loop? | (ZIP3 ?)3 | [43] |
| BIME1 (TTHERM_00460720) | Gene ID: 7825705, XP_001018768.3 | Y | Bivalent formation | — | [43] |
| MCMD1 (TTHERM_01207610) | Gene ID: 7836088, XP_001021907.2 | Y | MCM domain protein, chiasma formation | — | [49] |
| PAMD1 (TTHERM_001295283) | XM_012798783.1 | Y | Partner of Mcmd1, chiasma formation | [49] | |
| Meiotic divisions | |||||
| REC8 (TTHERM_00245660) | Gene ID: 7841491, XP_001023795.2 | N | Universal kleisin component of the cohesin complex | SCC1, REC8 | [59] |
| ESP1 (TTHERM_00297160) | Gene ID: 7834521, XP_001013249.2 | N | Separase | ESP1 | [59] |
TTHERM_—identifier of annotated genes in the TGD (http://ciliate.org/).
1Incorrect annotation in TGD; the transcript identifier (gene_) from the TFGD (http://tfgd.ihb.ac.cn/) is shown instead.
2EXO1 has a nonessential function in vegetative cells.
3Homology doubtful.
DSB, double-strand break; TFGD, Tetrahymena Functional Genomics Database; TGD, Tetrahymena Genome Database.