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. 2021 Jul 15;17(7):e1009544. doi: 10.1371/journal.ppat.1009544

Fig 2. ACE2 Decoy Binding and Neutralization Across Diverse CoVs.

Fig 2

A. Structural model (7DF4.pdb [42]) of Wuhan CoV-2 RBD (yellow) bound to human ACE2 (blue) RBD. The six RBD residues frequently mutated in emerging CoV-2 variants are shown in red spheres. B. Wuhan CoV-2 model as in (A) but with RBD residues highlighted at the ACE2 interface (red spheres) which differ from CoV-1. C. SPR measurements: ACE2-wt-Fc4 or CDY14HL-Fc4 binding to various purified recombinant RBD proteins. D. CDY14HL-Fc4 titrations using pseudotyped lentivirus reporters encoding CoV-2 spike proteins from four US-CDC Variants of Concern. RBD mutations are indicated in brackets, however in many cases other spike mutations exist that are not listed. E. Pseudotype titrations for six additional CoV-2 spike variants. G. Pseudotype titration for CoV-1 spike reporter virus. Reporter virus activity data are presented as mean ± standard error of the mean for at least three replicate titrations.