Fig 7. Loss of PGCs in MZigf2bp3^la659Tg mutant embryos might arise from cell death.

A. Some PGCs are lost during migration in maternal igf2bp3^la659Tg embryos. At bud stage, a subset of PGCs labelled with a GFP-nos1 live reporter display fragmentation during migration. B. Immunofluorescence with an α-Vasa antibody and TUNEL staining showed that compared to wild type embryos, in MZigf2bp3^la659Tg embryos a subset of PGCs undergo cell death and fragmentation at the bud stage. Yellow arrow indicates co-localisation of Vasa and TUNEL labelling in a PGC, white arrows indicate Vasa+ fragments; Scale bar, 50 μm.