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. 2021 Jun 4;12:679184. doi: 10.3389/fimmu.2021.679184

Figure 2.

Figure 2

RUVBL2 is essential for pro-inflammatory gene expressions. (A) Heatmap of pro-inflammatory gene expression using real-time PCR analysis at 4 and 24 hrs after LPS induction of RAW 264.7 macrophages transfected with siControl or SP-siRuvbl2. (B) Level of TNF-α, IFNγ, IL-6, IL-1β, and GM-CSF in culture medium of RAW 264.7 macrophages transfected with siControl or siRuvbl2, in the presence of LPS (10 ng/ml). (C) Kinetics of expression of representative primary response genes in LPS (10 ng/ml)-induced RAW 264.7 macrophages transfected with siControl or SP-siRuvbl2. (D) Kinetics of representative secondary response genes expression in siControl and SP-siRuvbl2 transfected cells in response to LPS (10 ng/ml). Data from (A–D) are obtained from three independent experiments and presented in mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, by unpaired t-test in (B) and by two-way ANOVA with Bonferroni’s multiple comparison test as post-test in (C, D).