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. 2021 Jul 1;9:609908. doi: 10.3389/fcell.2021.609908

FIGURE 3.

FIGURE 3

SV2A regulates the expression level of BACE1 and APOE. (A) qPCR detection of BACE1 mRNA expression level in APPswe293T cells from the following four groups: control, cells infected with the SV2A-overexpressing virus (SV2A), shControl, and cells infected with the SV2A-silencing virus (shSV2A). Gapdh was used as the internal control. (B) Western blotting detection of BACE1 expression levels in APPswe293T cells from the following four groups: control, cells infected with the SV2A-overexpressing virus (SV2A), shControl, and cells infected with the SV2A-silencing virus (shSV2A). The gray density was normalized to the mean gray density of Gapdh. (C) Immunofluorescence observation of the BACE1 (red) and SV2A (green) in APPswe293T cells. The nuclei were counterstained with DAPI (blue). Scale bar, 40 μm. (D) qPCR detection of the APOE mRNA expression level in APPswe293T cells from the following four groups: control, cells infected with the SV2A-overexpressing virus (SV2A), shControl, and cells infected with the SV2A-silencing virus (shSV2A). Gapdh was used as the internal control. (E) Western blotting detection of APOE expression levels in APPswe293T cells from the following four groups: control, cells infected with the SV2A-overexpressing virus (SV2A), shControl, and cells infected with the SV2A-silencing virus (shSV2A). The gray density was normalized to the mean gray density of Gapdh. For each group of APPswe293T cells, n = 3. Data are expressed as mean ± SEM. One-way analysis of variance (ANOVA). **p < 0.01.