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[Preprint]. 2021 Jul 5:2021.07.03.450938. [Version 1] doi: 10.1101/2021.07.03.450938

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Figure 1. — (A) Illustration of the HTRF assay for SARS-CoV-2 NP showing the Eu donor-conjugated primary MAb and DL650 acceptor-conjugated primary MAb detecting SARS-CoV-2 and enabling FRET. (B) Five MAbs specific for SARS-CoV-2 NP were labeled with Europium (donor Ab) or DyLight650^® (acceptor Ab). The labeled MAbs were then tested in HTRF assay, in triplicate wells using a cross-matrix assay format. The presented data is a median TR-FRET counts (acceptor fluorescence/donor fluorescence × 10,000). The MAb pair (R001-Eu/1C7-DyLight650) showed highest specific signal and was selected for further assay development.