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[Preprint]. 2021 Jul 5:2021.07.03.450938. [Version 1] doi: 10.1101/2021.07.03.450938

Figure 2. Optimization of donor to acceptor ratio, concentration, and incubation time using media or Vero E6 cells.

Figure 2.

TR-FRET ratio signal (acceptor/donor) detection using R001-Eu and 1C7-DL650 as the donor (D) and acceptor (A) pair respectively. SARS-CoV-2 NP was used to generate the 11-point standard curve starting from 1500 ng/mL serially diluted 1:2. HTRF reagents were incubated with NP in cell culture media only (A and B) or in media with 5000 Vero E6 cells (C and D) for either 1h at RT or O/N at 4°C. N = 3 wells per condition in a 384 well plate. Error bars indicate S.D.