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(A) Schematic representation of the BRET sensor, teluc-FUS231-P12-mCyRFP3, to detect S trimer. (B) Luminescence emission spectra and image of the BRET sensor (100 pM) in the presence (orange trace, 100 pM) and absence (blue trace) of S2P. Emission color change was observed using a mobile phone camera (inset top right). (C) Titration of S2P with 100 pM sensor protein (Mean ± SEM, n = 3 replicates from a single experiment).
