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. 2021 Jul 1;11:686479. doi: 10.3389/fcimb.2021.686479

Figure 2.

Figure 2

Biofilm growth of single, dual, and multispecies of S. sanguinis, C. durum, and P. gingivalis on Thermanox coverslips. (A) Bacterial biomass of biofilms grown on Thermanox coverslips for 3 days was visualized with crystal violet staining. A Thermanox coverslip incubated in medium only served as the negative control. Pictures are representative of 3 biological replicates. (B) Determination of total cell numbers using q-PCR. Biofilms grown on Thermanox coverslips were removed and bacterial DNA was isolated. The number of bacterial cells was calculated based upon standard curves of serially diluted DNA. Data represent the averages and standard deviations of 3 biological replicates. (p-value indicated). *p < 0.05 (Students t-test, two-tailed, paired).