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. 2021 Jul 1;11:681280. doi: 10.3389/fonc.2021.681280

Figure 6.

Figure 6

Identification of ITGB1 as FTO demethylated target gene in GC cells. (A, B) The relative mRNA and protein expression level of ITGB1 and LAMC1 in BGC823 and MGC803 cells after transfected with siNC or FTO siRNAs were detected by qRT-PCR and Western blot. (C) The m6A modification level of ITGB1 in BGC823 cell transfected with siNC or FTO siRNAs was detected by MeRIP-qRT-PCR. (D, E) The relative expression of ITGB1 in MGC803 and BGC823 cells after added with cycloleucine were detected by qRT-PCR and Western blot. (F) The relative expression of ITGB1 in BGC823 and MGC803 cells after co-transfected with siNC or FTO siRNAs and ITGB1 plasmids or empty vectors was detected by qRT-PCR. (G, H) The migration and invasion ability of BGC823 and MGC803 cells after co-transfected with siNC or FTO siRNAs and ITGB1 plasmids or empty vectors was examined by transwell assay (original magnification, 100×). The columns on the right are quantified by counting 3 fields, and presented as the mean ± standard deviation. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not statistically significant. 18S was used as an internal control for all qRT-PCR experiments. GAPDH was used as an internal control for all western blot assays.