Table 2.
Profiling expression of unique gene in lesions in WM clusters of healthy and SPMS braina.
| Protein | Gene | Neuron | Astrocyte | OPC | COP | ImOLG | Oligo | Microglia | Pericyte |
|---|---|---|---|---|---|---|---|---|---|
| K+ channels | |||||||||
| Kv1.1 | KCNA1 | n.d | n.d | n.d | n.d | n.d | n.d | n.d | n.d |
| Kv1.2 | KCNA2 | + | +/– | +/– | +/– | – | +/– | – | – |
| Kv1.4 | KCNA4 | +/– | – | – | – | – | – | – | – |
| Kv2.1 | KCNB1 | + | +/– | +/– | + | +/– | +/– | – | + |
| Kv2.2 | KCNB2 | ++ | +/– | – | +/– | +/– | +/– | – | – |
| Kv3.3 | KCNC3 | + | +/– | – | +/– | +/– | +/– | +/– | – |
| Kv4.2 | KCND2 | +++ | + | ++++ | +++ | + | +/– | +/– | +/– |
| Kv7.2 | KCNQ2 | + | +/– | + | + | +/– | +/– | – | +/– |
| Kv7.3 | KCNQ3 | +++ | + | + | + | ++ | +/– | +++ | +/– |
| Kv7.5 | KCNQ5 | ++++ | + | +/– | + | + | +/– | +/– | +/– |
| Kv8.1 | KCNV1 | + | – | – | +/– | +/– | – | – | – |
| Kv9.2 | KCNS2 | + | – | – | +/– | – | – | – | – |
| Kv10. 2/EAG2 | KCNH5 | + | +/– | +/– | + | +/– | +/– | – | – |
| Kv11.3/ERG3 | KCNH7 | +++ | +/– | – | + | + | +/– | – | – |
| Kv12.1/ELK1 | KCNH8 | + | +++ | ++ | +++ | ++ | +++ | +/– | +/– |
| TREK1 | KCNK2 | + | +/– | + | +/– | – | – | – | – |
| TREK2 | KCNK10 | + | +/– | +/– | + | +/– | +/– | – | – |
| KCa2.3 | KCNN3 | + | ++ | + | + | + | +/– | +/– | +/– |
| KNa1.1 | KCNT1 | + | – | – | +/– | +/– | – | – | – |
| Kir3.2 | KCNJ6 | + | +/– | + | + | – | + | – | – |
| Kir5.1 | KCNJ16 | – | +/– | + | + | – | – | – | – |
| Na+ channels | |||||||||
| Nav1.1 | SCN1A | ++ | + | +++ | ++ | + | +/– | – | – |
| Nav1.2 | SCN2A | +++ | + | +/– | + | + | +/– | – | +/– |
| Nav1.3 | SCN3A | ++ | +/– | ++ | ++ | + | + | – | +/– |
| Nav1.6 | SCN8A | n.d | n.d | n.d | n.d | n.d | n.d | n.d | n.d |
| Nav1.9 | SCN11A | n.d | n.d | n.d | n.d | n.d | n.d | n.d | n.d |
| Ca2+ channels | |||||||||
| Cav1.2 | CACNA1C | +++ | + | + | + | + | +/– | – | +++ |
| Cav1.3 | CACNA1D | ++ | +/– | + | + | + | +/– | + | – |
| Cav2.1 | CACNA1A | +++ | + | +++ | ++ | + | +/– | + | +/– |
| Cav2.3 | CACNA1E | ++ | +/– | +/– | + | + | +/– | – | – |
| Cav3.1 | CACNA1G | + | – | +/– | +/– | – | – | – | – |
| Cav3.2 | CACNA1H | n.d | n.d | n.d | n.d | n.d | n.d | n.d | n.d |
| Cav3.3 | CACNA1I | + | – | – | +/– | – | – | – | – |
| Ryanodine | |||||||||
| Ryr2 | RYR2 | ++++ | + | +/– | + | + | + | +/– | + |
| Ryr3 | RYR3 | + | +++ | + | + | + | +/– | +/– | +/– |
| TRP channels | |||||||||
| TRPM2 | TRPM2 | + | +/– | – | +/– | + | – | + | +/– |
| TRPP1 | PKD2 | + | ++ | + | ++ | ++ | + | + | + |
| TRPP3 | PKD2L2 | n.d | n.d | n.d | n.d | n.d | n.d | n.d | n.d |
| TRPV1 | TRPV1 | +/– | +/– | – | +/– | – | +/– | – | – |
| TRPV6 | TRPV6 | n.d | n.d | n.d | n.d | n.d | n.d | n.d | n.d |
| Cl− channels | |||||||||
| CLC−2 | CLCN2 | +/– | +/– | +/– | +/– | +/– | + | – | – |
| CLC−7 | CLCN7 | + | + | + | + | + | + | + | +/– |
| Connexins | |||||||||
| Cx37 | GJA4 | – | – | – | – | – | – | – | ++ |
| Pannexin | |||||||||
| Px1 | PANX1 | n.d | n.d | n.d | n.d | n.d | n.d | n.d | n.d |
| Catsper | |||||||||
| CATSPERG | CATSPERG | +/– | +/– | – | +/– | +/– | +/– | – | – |
| CATSPERE | CATSPERE | n.d | n.d | n.d | n.d | n.d | n.d | n.d | n.d |
sn-RNAseq from the white matter of individuals with SPMS and non-neurological controls. The information is based on the snRNAseq from the WM of individuals with SPMS and non-neurological control subjects (Jakel et al., 2019), and data are collected from the database available at https://ki.se/mbb/oligointernodeen/. Expression levels are based on the mean normalized expression counts (log-scale) per cluster.
+/– (log scale > 0.01 ≤ 0.1); + (log scale > 0.1 ≤ 0.5); ++ (log scale > 0.6 ≤ 1); +++ (log scale >1.1 ≤ 1.5); ++++ (log scale > 1.5); – (log scale 0); n.d., not detected; red (+), highly expressed gene in the cluster if compared to the rest of the clusters.
Note, that in the database, some of the clusters encompass both control and MS samples and, therefore, the mean can represent a combination of counts from control and MS brain.