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Examples in two control mice of the effects of an injection of fentanyl (up to 5 mg/kg ip in 1 mouse and at 60 mg/kg ip in another mouse of same weight) on instantaneous respiratory flow (V̇). Note that the depression in breathing produced by fentanyl was “interrupted” by bursts of locomotor activity (horizontal lines) during which ventilation was much higher than in baseline condition when the animal was immobile.
