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. 2021 Jul 1;12:698185. doi: 10.3389/fphys.2021.698185

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Copyright © 2021 Fan, Jiang, Gao, Bigalke, Chen, Yu, Chen and Shan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ICV injection of orexin-A increases mRNA expression of CaMKIIa and CamKIIb in the PVN of SD rats. Adult male SD rats received ICV injection of either orexin-A or vehicle control. The rats were euthanized 3 h following microinjection, and PVN tissue was harvested and subjected to real-time PCR to measure mRNA expression of CaMKIIa (A) and CaMKIIb (B). The data were normalized to GAPDH mRNA and the average mRNA levels in control rats were assigned an arbitrary unit of 1 (n = 5/group). P = 0.006 for the CaMKIIa mRNA (A) increase and P = 0.022 for the CaMKIIb mRNA (B) increase. * P < 0.05.