TABLE 1.
Antibodies | Company | Catalog number | Source | Dilutions for WB | Dilution for IF |
Primary antibodies | |||||
OX1R | Acris | Ap31067pu-n | Goat | / | 1:50 |
OX1R | Alomone labs | AOR-001 | Rabbit | / | 1:100 |
NeuN | Cell Signaling | mAb#24307 | Rabbit | / | 1:50 |
Iba1 | Wako | 019-19741 | Rabbit | / | 1:500 |
GFAP | Invitrogen | mAb#3670 | Mouse | / | 1:300 |
Anti-pCaMKII | Santa cruz | sc-32289 | Mouse | 1:1000 | 1:200 |
CaMKIIa | Santa cruz | sc-13141 | Mouse | 1:1000 | / |
CaMKIIb | Santa cruz | sc-376828 | Mouse | 1:1000 | / |
β-actin | Santa cruz | sc-47778 | Mouse | 1:1000 | / |
Secondary antibodies | |||||
Anti-goat 488 | Invitrogen | A32814 | Donkey | / | 1:1000 |
Anti-rabbit 594 | Invitrogen | A-21207 | Donkey | / | 1:1000 |
Anti-rabbit 488 | Invitrogen | A-21206 | Donkey | / | 1:1000 |
Anti-mouse 594 | Invitrogen | A-11005 | Goat | / | 1:1000 |
Anti-mouse IgG (HRP) | Invitrogen | PA1-28568 | Rabbit | 1 :2000 | / |
WB, western blot; IF, immunofluorescence.