FIGURE 2.
Pharmacological inhibition of IKKα and IKKβ prevents senescence mediated by the evSASP (a–c) HFFF2 recipient cells were treated with 20 µM CAY10576, 10 µM MLN120B or 10 µM BAY11‐7082 in addition to sEV isolated from iRAS cells for 6 days. The percentage of cells staining positive for (a, b) BrdU, SA‐β‐Gal and (c) p16INK4A was determined. (a) Quantification of the percentage of cells staining positive for BrdU and SA‐β‐Gal and (b) representative pictures. (c) Quantification and pictures for p16INK4A staining by IF. (a, c) Data show the mean ± SEM of 3 independent experiments. One‐way ANOVA with multiple comparisons with the DMSO/sEV from iRAS sample was performed. Scale bar: 50 µm