FIGURE 3.

Knockout of IKKα, IKKβ and IKKε using sgRNA prevents senescence mediated by the evSASP (a) Representation showing the experimental settings where HFFF2 recipient cells expressing or not different sgRNA targeting IKKα (sgIKKA), IKKβ (sgIKKB), or IKKε (sgIKKE) were incubated with sEV from iC or iRAS for 6 days. (b–d) HFFF2 recipient cells transduced with sgIKKA, sgIKKB or sgIKKE were treated with sEV isolated from iC or iRAS cells for 6 days. The percentage of cells and representative pictures staining positive for (b) BrdU, (c) SA‐β‐Gal (d) and p16^INK4A is shown. Data show the mean ± SEM of 3 independent experiments. One‐way ANOVA with multiple comparisons to the C/sEV from iRAS sample was performed. Scale bar: 50 µm