FIGURE 6.

IKKs are important for evSASP senescence in human primary fibroblasts from young and old donors. (a) Schematic representation of the experimental settings where 4 human fibroblasts cell cultures isolated from young, Y, (~2 years) and old, O, (~70 years) donors were used. Pooled sEV isolated from four old cell cultures were used to treat four young cell cultures individually expressing and empty vector or sgRNAs targeting IKKs. (b, c) Relative mRNA levels for (b) the cell cycle regulators CDKN1A and CDKN2A or (c) components of the sSASP IL8 and IL6 were determined. All data represents the mean ± SEM of four individual cell cultures. One‐way ANOVA with Dunnett's multiple comparison analysis to control sEV old treated sample was performed. Data are normalized to the control sEV from the old donors sample