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. 2021 Jun 9;20(7):e13417. doi: 10.1111/acel.13417

FIGURE 3.

FIGURE 3

Loss of α‐Klotho‐induced premature senescence in JAR cells. (a) Western blotting of α‐Klotho in JAR cells. Sh‐NC, negative control cells transfected with scramble shRNA; Sh‐KL#1‐3, cells transfected with different shRNAs targeting α‐Klotho; (b) Western blotting of α‐Klotho protein expression in JAR cells. WT, wild‐type; OE‐NC, negative control; and OE‐KL, α‐Klotho overexpression; n = 3. (c) Representative SA‐β‐Gal staining in various JAR cells. Scale bars, 200 μm; (d) Western blotting of p53, p21, and p16 protein expression in Sh‐KL and OE‐KL JAR cells; (e) transwell invasion assay of JAR cells; (f) apoptosis was assessed using flow cytometry, n = 3. All data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, one‐way ANOVA. NS, nonsignificant. All experiments were performed in triplicate.