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. 2021 Jun 5;20(7):e13411. doi: 10.1111/acel.13411

FIGURE 5.

FIGURE 5

Gene expression analyses of human myogenic markers over time following engraftment. (a‐d) Relative gene expression reported as normalized fold‐change for human‐specific myogenic mRNA transcripts PAX7 (a), MYOD (b), MYOG (c), and MYH3 (d) for xenograft samples collected 3‐ and 6‐week post‐engraftment. Raw qRT‐PCR data were normalized to human‐specific house keeping gene, HRPT, and non‐regenerated human negative control muscle to calculate ΔCt and ΔΔCt, respectively. Normalized gene expression fold‐change was calculated using the formula, 2−(ΔΔ C t). Statistical analyses performed by two‐tailed, non‐parametric, Mann–Whitney test; ns, not significant; *p < 0.05; **p < 0.01.