FIGURE 6.

Nucleotide‐binding domain and leucine‐rich‐repeat‐containing protein X1‐dependent alterations of mTOR, p53, and p16^INK4A in murine lungs. (a) Immunoblot to detect NLRX1, mTOR, and phosphorylation of mTOR at serine 2448 (p‐mTOR^S2448) in lungs from 6‐ or 16‐month‐old (mo) wild‐type (WT) mice. (b) Immunoblot to detect NLRX1, mTOR, and p‐mTOR^S2448 in lungs from 6‐months WT or NLRX1 knockout (KO) mice. (c) Densitometry evaluation of the relative mTOR or p‐mTOR^S2448 protein expression levels. n = 3 mice, each group. (d) Immunoblot to evaluate the phosphorylation status of p53 at serine 15 (p‐p53^S15) in lungs of 6‐ or 16‐months WT and (e) 6‐months WT or NLRX1 KO mice. (f) Densitometry evaluation of the relative p‐p53^S15 protein expression levels. n = 3 mice, each group. (g) Immunoblot to detect SIRT1 and p16^INK4A in lungs from 16‐month‐old (mo) wild‐type (WT) or NLRX1 knockout (KO) mice. (h) Graph showing the relative SIRT1 and (i) p16^INK4A protein expression levels. n = 7 mice, each group. β‐actin used as a loading control. Error bars indicate means ± SEM. Data were analyzed by a two‐tailed unpaired t test. ***p < 0.005, **p < 0.01, *p < 0.05