FIGURE 2.

The effect of TRIM33 on FOXO3a expression in H₂O₂‐induced osteoblasts. (a) HOB and MC3 T3‐E1 cells were co‐transfected with Lv‐TRIM33 (or Lv‐GFP) for 24 h, followed by H₂O₂ (50 μM) treatment for another 24 h. The protein levels of TRIM33, nuclear and total FOXO3a were tested by Western blot. (b) HOB and MC3 T3‐E1 cells were transfected with Lv‐TRIM33 or Lv‐GFP for 24 h and then treated with H₂O₂ (50 μM) for another 24 h. Later, cells were treated with CHX for indicated times (0, 1, 2, and 4 h). The protein level of FOXO3a was tested by Western blot. ^* p < 0.05, ^** p < 0.01 vs. H₂O₂ + Lv‐GFP group. The experiment was repeated three times