FIGURE 3.
Effect of TRIM33 overexpression on the acetylation of FOXO3a. (a) The binding capacity of TRIM33 to CBP in HOB and MC3 T3‐E1 cells was tested by IP assay. (b) HOB and MC3 T3‐E1 cells were transfected with Lv‐TRIM33 or Lv‐GFP and then the binding capacity of TRIM33 to CBP in HOB and MC3 T3‐E1 cells was tested by IP assay. (c) HOB and MC3 T3‐E1 cells were transfected with Lv‐CBP and/or Lv‐TRIM33 . The acetylation of FOXO3a in cells was tested by IP assay and the protein levels of TRIM33 and CBP were tested by Western blot. (d) HOB and MC3 T3‐E1 cells were transfected with Lv‐TRIM33 or Lv‐GFP for 24 h and then the cells were treated with H2O2 (50 μM) for another 24 h. The acetylation of FOXO3a in cells was tested by IP assay. The experiment was repeated three times