FIGURE 6.

Effect of TRIM33 overexpression on osteoporosis of OVX mice. Mice were subjected to construct osteoporosis model by OVX operation and injected with AAV9‐TRIM33 or AAV9‐GFP by the tail vein, and seven mice were assigned to each group. Eight weeks after the model establishment, all the mice were sacrificed. (a, b) Then BMD and BMC of mouse femurs. (c) The image of Micro‐CT scanning of mouse femurs. Scale bars = 500 μm. (d–g) The BV/TV, trabecular number (Tb. N), trabecular thickness (Tb. Th), and trabecular spacing (Tb. Sp) of the femurs. (h) The concentration of PINP in serum. (i) The concentration of TRACP‐5b in serum. (j) The protein levels of TRIM33 and FOXO3a in the osteoblasts isolated from mouse bone marrow were tested by Western blot. (k) The detection of immunostained for RUNX2. (l) The detection of estradiol (E2) level in the serum samples. ^* p < 0.05, ^** p < 0.01 vs. Sham+AAV9‐GFP group; ^# p < 0.05 vs. OVX+AAV9‐GFP group. n = 7