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SASP is induced by overexpression of hCLCA1 in proximal tubular epithelial cells in vitro. (a‐c) Overexpression of hCLCA1 resulted in increase in the expression of p53, p21, and p16INK4a. (d‐f) Increased expression of IL‐1α, IL‐1β, and IL‐6 was seen in cells overexpressing hCLCA1. Data (mean ± SD) are shown in bars with scatter plots and were analyzed by t test. *p < 0.05, **p < 0.01, ***p < 0.001. (a‐f, five control and six hCLCA1 clones were employed)
