FIGURE 5.
TMEM16A inhibition abolishes changes in fibronectin and SASP induced by overexpression of hCLCA1 in proximal tubular epithelial cells in vitro. (a‐c) T16Ainh‐A01, a selective TMEM16A inhibitor, inhibited increase in matrix fibronectin induced by hCLCA1 overexpression versus vector‐transfected controls without affecting the expression of CLCA1 and TMEM16A. (d‐h) T16Ainh‐A01 abolished hCLCA1‐overexpression‐induced increase in p53, p21, p16INK4a, IL‐1β, and IL‐6. Data from 3–4 experiments (mean ± SD) are shown in scatter plots and were analyzed by ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001