FIGURE 4.

Inability to upregulate eNOS expression in HGPS iPSC‐ECs under shear stress. (a). Schematic illustration of the experimental setup of the fluidic culture chamber (left), and shear stress distribution on inner chamber surfaces from fluid flow simulation (right). (b) Immunofluorescence staining of eNOS in normal and HGPS iPSC‐ECs under static and fluidic culture conditions (scale bars = 200 μm). (c) Quantification of the eNOS fluorescence signal intensity. (d) Total intracellular NO level using a colorimetric assay in normal and HGPS ECs in the fluidic chamber. Data are presented as mean ± SEM, **p < 0.01; ***p < 0.001; n, three independent experiments